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recombinant soluble human rantes  (R&D Systems)


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    R&D Systems recombinant soluble human rantes

    Recombinant Soluble Human Rantes, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 42 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/recombinant soluble human rantes/product/R&D Systems
    Average 92 stars, based on 42 article reviews
    recombinant soluble human rantes - by Bioz Stars, 2026-02
    92/100 stars

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    1) Product Images from "Derived myeloid lineage induced pluripotent stem as a platform to study human C-C chemokine receptor type 5Δ32 homozygotes"

    Article Title: Derived myeloid lineage induced pluripotent stem as a platform to study human C-C chemokine receptor type 5Δ32 homozygotes

    Journal: iScience

    doi: 10.1016/j.isci.2023.108331


    Figure Legend Snippet:

    Techniques Used: Virus, Recombinant, Modification, Purification, Blocking Assay, Luminex, Pore Size, Amplification, Software



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    recombinant soluble human rantes  (R&D Systems)
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    R&D Systems recombinant soluble human rantes

    Recombinant Soluble Human Rantes, supplied by R&D Systems, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
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    soluble recombinant–human rantes (rh-rantes)  (PeproTech)
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    PeproTech soluble recombinant–human rantes (rh-rantes)
    Functionality of primary and iPSC-derived macrophages Phagocytic ability of M0, M1 and M2 primary macrophages (pMac, white bar, n = 4) and iPSC-derived macrophages (iMac) obtained from both controls (iMac-Con, light gray bar; n = 3) and individuals homozygous for CCR5Δ32 variant (iMac-CCR5Δ32, dark gray bar; n = 4) was evaluated by using pHrodo Green Zymosan A Bioparticles and was assessed by flow cytometry and confocal microscopy. (A) Macrophage phagocytic ability assessed by flow cytometry was shown as frequency of Zymosan+ cells. (B) Representative photographs showing phagocytized zymosan particles (in green) in primary macrophages (pMac, first row, n = 3) and iMac obtained from healthy controls (iMac-Con, second row, n = 3) and iMac obtained from homozygous CCR5Δ32 individuals (iMac-CCR5Δ32, third row, n = 4) polarized in M0, M1, and M2 macrophages (First, second and third column, respectively). Nuclei were stained with DAPI (blue). Scale bar: 20 μm. (C) The chemotactic ability of primary monocytes (pMono, white circle; n = 3) and iPSC-derived monocytes (iMono) obtained from healthy controls (iMono-Con, light gray square; n = 3) and from homozygous CCR5Δ32 individuals (iMono-CCR5Δ32, dark gray square; n = 3) to migrate in response to increasing the concentration of <t>RANTES</t> was assessed in vitro by a cell migration assay. The number of migrated cells was evaluated at different time points. ∗ iMono-Con vs. iMono-CCR5Δ32; # pMono-Con vs. iMono-CCR5Δ32. (D and E) Macrophage infectability with HIV was assessed in M1 and M2 polarized primary and iPSC-derived macrophages obtained from healthy controls (iMac-Con) and from homozygous CCR5Δ32 individuals (iMac-CCR5Δ32) by evaluating through qPCR the presence of (D) specific HIV-1 DNA Gag and (E) specific HIV-1 DNA LTR RU5. Amplification of genomic GAPDH as a reference gene was used to control the amount of DNA in each sample. Data shown as mean ± SEM. Statistical significance was calculated using ANOVA. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗∗p < 0.0001.
    Soluble Recombinant–Human Rantes (Rh Rantes), supplied by PeproTech, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/soluble recombinant–human rantes (rh-rantes)/product/PeproTech
    Average 90 stars, based on 1 article reviews
    soluble recombinant–human rantes (rh-rantes) - by Bioz Stars, 2026-02
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    		  Buy from Supplier

    Image Search Results


    Journal: iScience

    Article Title: Derived myeloid lineage induced pluripotent stem as a platform to study human C-C chemokine receptor type 5Δ32 homozygotes

    doi: 10.1016/j.isci.2023.108331

    Figure Lengend Snippet:

    Article Snippet: In the lower part of the transwell, DMEM medium (Lonza) was added with recombinant–soluble human RANTES (rh-RANTES; R&D) that was used as an attractant chemokine at different concentrations (1, 3, and 10 ng/ml).

    Techniques: Virus, Recombinant, Modification, Purification, Blocking Assay, Luminex, Pore Size, Amplification, Software

    Functionality of primary and iPSC-derived macrophages Phagocytic ability of M0, M1 and M2 primary macrophages (pMac, white bar, n = 4) and iPSC-derived macrophages (iMac) obtained from both controls (iMac-Con, light gray bar; n = 3) and individuals homozygous for CCR5Δ32 variant (iMac-CCR5Δ32, dark gray bar; n = 4) was evaluated by using pHrodo Green Zymosan A Bioparticles and was assessed by flow cytometry and confocal microscopy. (A) Macrophage phagocytic ability assessed by flow cytometry was shown as frequency of Zymosan+ cells. (B) Representative photographs showing phagocytized zymosan particles (in green) in primary macrophages (pMac, first row, n = 3) and iMac obtained from healthy controls (iMac-Con, second row, n = 3) and iMac obtained from homozygous CCR5Δ32 individuals (iMac-CCR5Δ32, third row, n = 4) polarized in M0, M1, and M2 macrophages (First, second and third column, respectively). Nuclei were stained with DAPI (blue). Scale bar: 20 μm. (C) The chemotactic ability of primary monocytes (pMono, white circle; n = 3) and iPSC-derived monocytes (iMono) obtained from healthy controls (iMono-Con, light gray square; n = 3) and from homozygous CCR5Δ32 individuals (iMono-CCR5Δ32, dark gray square; n = 3) to migrate in response to increasing the concentration of RANTES was assessed in vitro by a cell migration assay. The number of migrated cells was evaluated at different time points. ∗ iMono-Con vs. iMono-CCR5Δ32; # pMono-Con vs. iMono-CCR5Δ32. (D and E) Macrophage infectability with HIV was assessed in M1 and M2 polarized primary and iPSC-derived macrophages obtained from healthy controls (iMac-Con) and from homozygous CCR5Δ32 individuals (iMac-CCR5Δ32) by evaluating through qPCR the presence of (D) specific HIV-1 DNA Gag and (E) specific HIV-1 DNA LTR RU5. Amplification of genomic GAPDH as a reference gene was used to control the amount of DNA in each sample. Data shown as mean ± SEM. Statistical significance was calculated using ANOVA. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗∗p < 0.0001.

    Journal: iScience

    Article Title: Derived myeloid lineage induced pluripotent stem as a platform to study human C-C chemokine receptor type 5Δ32 homozygotes

    doi: 10.1016/j.isci.2023.108331

    Figure Lengend Snippet: Functionality of primary and iPSC-derived macrophages Phagocytic ability of M0, M1 and M2 primary macrophages (pMac, white bar, n = 4) and iPSC-derived macrophages (iMac) obtained from both controls (iMac-Con, light gray bar; n = 3) and individuals homozygous for CCR5Δ32 variant (iMac-CCR5Δ32, dark gray bar; n = 4) was evaluated by using pHrodo Green Zymosan A Bioparticles and was assessed by flow cytometry and confocal microscopy. (A) Macrophage phagocytic ability assessed by flow cytometry was shown as frequency of Zymosan+ cells. (B) Representative photographs showing phagocytized zymosan particles (in green) in primary macrophages (pMac, first row, n = 3) and iMac obtained from healthy controls (iMac-Con, second row, n = 3) and iMac obtained from homozygous CCR5Δ32 individuals (iMac-CCR5Δ32, third row, n = 4) polarized in M0, M1, and M2 macrophages (First, second and third column, respectively). Nuclei were stained with DAPI (blue). Scale bar: 20 μm. (C) The chemotactic ability of primary monocytes (pMono, white circle; n = 3) and iPSC-derived monocytes (iMono) obtained from healthy controls (iMono-Con, light gray square; n = 3) and from homozygous CCR5Δ32 individuals (iMono-CCR5Δ32, dark gray square; n = 3) to migrate in response to increasing the concentration of RANTES was assessed in vitro by a cell migration assay. The number of migrated cells was evaluated at different time points. ∗ iMono-Con vs. iMono-CCR5Δ32; # pMono-Con vs. iMono-CCR5Δ32. (D and E) Macrophage infectability with HIV was assessed in M1 and M2 polarized primary and iPSC-derived macrophages obtained from healthy controls (iMac-Con) and from homozygous CCR5Δ32 individuals (iMac-CCR5Δ32) by evaluating through qPCR the presence of (D) specific HIV-1 DNA Gag and (E) specific HIV-1 DNA LTR RU5. Amplification of genomic GAPDH as a reference gene was used to control the amount of DNA in each sample. Data shown as mean ± SEM. Statistical significance was calculated using ANOVA. ∗p < 0.05; ∗∗p < 0.01; ∗∗∗∗p < 0.0001.

    Article Snippet: Soluble recombinant–human RANTES (rh-RANTES) , PeproTech , 300-06.

    Techniques: Derivative Assay, Variant Assay, Flow Cytometry, Confocal Microscopy, Staining, Concentration Assay, In Vitro, Cell Migration Assay, Amplification, Control

    Journal: iScience

    Article Title: Derived myeloid lineage induced pluripotent stem as a platform to study human C-C chemokine receptor type 5Δ32 homozygotes

    doi: 10.1016/j.isci.2023.108331

    Figure Lengend Snippet:

    Article Snippet: Soluble recombinant–human RANTES (rh-RANTES) , PeproTech , 300-06.

    Techniques: Virus, Recombinant, Modification, Purification, Blocking Assay, Control, Library Quantification, Luminex, Pore Size, Amplification, Software